Objective To  analyze the clinicopathological features of gallbladder adenosquamous carcinoma.Methods The clinical data of 8 patients with gallbladder adenosquamous carcinoma who were admitted to the Peking Union Medical College Hospital from January 2000 to December 2010 were retrospectively analyzed. The clinicopathological features, diagnosis and treatment strategies were summarized. The survival curve was drawn by Kaplan Meier method, and the survival of the patients was analyzed by Log rank test. Results The clinical manifestation of the 8 patients was non specific, and all the patients were misdiagnosed as with primary gallbladder cancer complicated with cholecystolithiasis or chronic cholecystitis. The levels of carcinoembryonic antigen and γ glutamyl transferase of 3 patients were higher than the normal, the level of alkaline phosphatase of 2 patients was higher than the normal, the level of aspartate transaminase of 1 patient was higher than the normal, the level of CA242 of 3 patients was higher than the normal and the level of CA19 9 of 4 patients was higher than the normal. The levels of aspartate transaminase, total bilirubin and direct bilirubin were in the normal range. Different surgical approaches were selected according to different tumor positions. The mean diameter of the tumor was (5±3)cm. Lymph node metastasis was detected at the hepatic hilar, hepatoduodenal ligament and area behind pancreatic head. Postoperative pathological examination confirmed that all the patients were with gallbladder adenosquamous carcinoma. All patients were followed up till April 2011. Two patients survived with no discomfort, and the survival time was 2 3 months. Six patients died of tumor recurrence or metastasis, and their median survival time was 8 months (range, 4 13 months). Conclusions The clinical manifestation of gallbladder adenosquamous carcinoma is non specific, and definite diagnosis depends on the results of postoperative pathological examination. Surgical treatment is the only effective method in improving the prognosis of patients with gallbladder adenosquamous carcinoma, and the prognosis of patients is poor.